Chemiluminescencyjna metoda oznaczania stężenia adenozyny i jej metabolitów w mikrodializatach tkankowych
Adenosine is one of regulative factors affecting renal function. It is considered that disturbances of kidney function in some pathophysiological conditions are the result of disturbances in adenosine signaling, resulting from changes of its concentration in the extracellular liquid. Measurement of adenosine concentration in the interstitial liquid of the kidney in some pathophysiological states can contribute to the better recognition of the role of extracellular adenosine in the pathogenesis of kidney function disturbances. Accordingly, the chemiluminescent method of adenosine determination in microdialysate samples of rat renal cortex was initiated. Factors interfering in adenosine measurement – uric acid and inosine, hypoxanthine and xanthine were identified. The interference of uric acid was limited by the incubation of samples with the uricase, the enzyme degrading uric acid to allantoin. The method of pattern addition was used, what enabled the qualification of adenosine in dialysates, in the range of concentrations from 50 to 400 nmol/l, with precision defined by mean coefficient of variation at 23%.
The chemiluminescence method allows to determinate adenosine in the interstitial liquid of the kidney cortex received by microdialysis. The incubation of dialysate with uricase enables the usage of this method for determination of adenosine in samples containing uric acid. The method of pattern addition eliminates the interference of unknown matters present in the dialysate.
Adres: mgr Robert Kowalski
Samodzielna Pracownia Terapii Monitorowanej i Farmakogenetyki AMG
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