Niestabilność mikrosatelitarna w przewlekłych zapalnych chorobach jelita grubego i w raku jelita grubego
P. M. Wierzbicki, K. Adrych, D. Kartanowicz, J. Wypych, M. Stanisławowski, S. Dobrowolski, J. Chybicki, M. Zwolińska-Wcisło, K. Celiński, B. Korybalski, M. Smoczyński, Z. Śledziński, Z. Kmieć
Microsatellite instability (MSI) is caused by damaged cellular DNA repairing system. MSI analysis in colorectal cancer (CRC) and in long-standing inflammatory bowel disease (IBD) is useful to differentiate various molecular pathways between MSI and MSS (microsatellite stable) cancers. AIM: To check the occurrence of MSI in DNA of CRC and IBD patients on the basis of microsatellite marker reference panel. MATHERIALS and METHODS: DNA was isolated from inflamed/cancer biopsies as well as venous blood of 57 CRC patients, 30 active ulcerative colitis (UC), 9 Crohn’s disease (CD) and 9 UC-associated dysplastic lesions. PCR reactions were performed using primers of BAT26, BAT25 and BAT40 markers, followed by denaturating polyacrylamide electrophoresis. RESULTS: MSI-H (mutation in all markers) was found in 1/57 (1.7%), whereas MSI-L was found in 5/57 (8.7%) of CRC patients. The highest occurrence of mutations was revealed in BAT26 marker – 6/57 (10.5%). We found no statistically significant relationships between MSI status and clinical and histopathological data in CRC. None MSI was found in UC, CD and UC-associated dysplastic lesions. CONCLUSION: MSI assessment based on 3 microsatellite reference markers suggest small participation of this process in the development of CRC and its minimal role in IBD in the studied groups.